The Quick-Tissue Series

Speedy and Simple

The Quick-Tissue™ Series is a complete reagent set that performs the fastest differentiation of induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs). For example, Elixirgen Scientific’s Quick-Neuron™ Cholinergic kit performs faster than any of the protocols used by researchers and other companies today. ESCs/iPSCs become functionally mature neurons in only one week. Such speed does not come at the cost of efficiency – past data shows that up to 90% of iPSCs and ESCs differentiate into cholinergic neurons.

Functional

Elixirgen Scientific’s Quick-Neuron™ cells fire strong and unique synchronized bursts when they are measured using multielectrode array (MEA; see below). Robust compound-specific dose responses were observed on MEA using our mixed neuron product. A wide variety of neurons derived from healthy individuals as well as disease patients are available.

Use Any Cells

With the Quick-Tissue™ kits, researchers can use cells of their choice and differentiate them into specific cell types. For example, researchers can use human ES cells and human iPS cells from a variety of sources or cells that they have generated by themselves. In addition, a large number of human iPS cells derived from patients with many different kinds of diseases are becoming available from the California Institute for Regenerative Medicine through the Coriell Biorepository. It has also become popular to make specific mutations in human ES and iPS cells by using genome editing technologies such as CRISPR/Cas9. Researchers can use any of these human ES and iPS cells and differentiate them to specific cell types for their own studies.

Genome-corrected iPS cells

Scalable

The The Quick-Tissue™ Series reagents are composed of cocktails of transcription factors, and thus, they are available in different sizes and scales. Elixirgen Scientific offers small-scale differentiation kits with reagents sufficient to differentiate cells in 4 wells of 24-well microtiter plate. For toxicity screening, Elixirgen Scientific offers a medium size differentiation kit sufficient to differentiate cells in 1x 96-well microtiter plates. For drug screening and other large-scale screening purposes, Elixirgen Scientific offers larger sizes differentiation kits sufficient to differentiate cells in 100x 96-well microtiter plates.

No Footprint

Thanks to its basis in RNA technology or Sendai Virus technology, the The Quick-Tissue™ Series treatment does not leave behind a genetic footprint in the cells it differentiates, unlike many common differentiation strategies. This ensures that your experiments with subjects such as disease iPSCs will not be unduly modified during the differentiation process.

Quick-Tissue™ Series Kits with RNA

The The Quick-Tissue™ Series kits not marked as “SeV” use advanced RNA technology in order to differentiate your cells without a trace. Since RNA is transcribed in the cytoplasm then used up, there will not be gene expression beyond what is required, and the kit’s RNA will be consumed in order to enact the differentiation.

Quick-Tissue™ Series Kits with Sendai Virus

The The Quick-Tissue™ Series SeV kits partially rely on the Sendai Virus (SeV) to cause differentiation for its subject cells. SeV is a RNA-virus – none of the phases of its life cycle involve DNA, so there is little risk of genomic integration. In addition, the particular type of SeV used by Elixirgen Scientific’s kits is temperature sensitive; it is most active at 33 degrees C, then becomes neutralized once its temperature reaches 37 degrees C. This neutralization successfully eliminates the virus from the cells.