FAQ | Quick-Tissue human Pluripotent Stem Cell Differentiation Kits

My culture has colonies that appear balled up and round on Day 1.

Coating Material A was diluted at room temperature or diluted using PBS warmed to room temperature. Keep both Coating Material A and PBS on ice before dilution.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Protocol, Coating, Plating, All differentiation kits

My culture did not survive or had many floating cells/cellular debris on Day 1.

hPSCs were mechanically harvested by scraping and so damaged. Lift Solution D1-treated iPSCs only by pipetting them up and down up to 15 times.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Protocol, Plating, Cell death, All differentiation kits

It was difficult to dissociate my source hPSC culture with Solution D1 without scraping them.

The concentration of the substrate used to coat the dish/well was too high. Reduce the concentration of the substrate to 70% or 50%.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Protocol, Plating, All differentiation kits

I plated cells as instructed but the confluency of my culture looked less than the one shown in the protocol on Day 1.

hPSCs were damaged while they were harvested. Ideally hPSCs should be lifted only by pipetting several times using Solution D1 as instructed in the protocol. If pipetting requires more than 15 times, reduce the concentration of the substrate used to coat the dish/well for the maintenance of the source hPSCs to 70% or 50%.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Protocol, Plating, All differentiation kits

My culture was seeded only in the center but not in the periphery of the well. What should I do?

We have observed that 4-well plates available from Nunc sometimes do not allow cells to seed in the periphery of the well. Next time, please try the following: 1. switch to 24-well plates; 2. incubate the plate with Coating Material A at 4°C overnight, instead of 37°C for 2 hours; 3. do not touch the bottom of wells with fingers when handling the plate.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Protocol, Plating, All differentiation kits

The component in my kit has a less volume than it is supposed to be.

All reagents in our kits are provided 10% more than needed. Please check if pipettors used are calibrated recently. On the other hand, particularly for the components and the coating materials, a small volume may have been on a tube wall. Breif centrifugation of those tubes will bring the whole content to the bottom. If pipettors have been calibrated recently and the volume is less than it is supposed to be even after the tube is spun down, please contact our customer support by phone (+1-443-869-5420) or email ([email protected]).

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Components, All differentiation kits

I am a novice in manipulating the fate of hPSCs. Which one of the kits should I use, an mRNA-based or a SeV-based kit?

SeV-based kits are suited better for customers who have experience in cell culture in general but have primitive experience in hPSC culture. However, our mRNA-based kits are simple enough to handle: it only requires two serial treatments and medium changes a day for the first two days.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Kit selection, All differentiation kits

Is there any difference in the differentiation efficiency between an mRNA-based and a SeV-based kit?

Based on our data, for example, the percentages of TUBB3+neurons differentiated using Quick-Neuron™ Cholinergic mRNA and SeV were 93% and 88%, respectively. Thus, there is not much differences in the differentiation efficiency between an mRNA-based and a SeV-based kit.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Kit selection, All differentiation kits

How long do I need to maintain hPSCs after plating prior to the application to your kit?

Unlike other human cells, hPSCs form disc-shaped colonies with tightly packed cells if their pluripotency is maintained. Thus, it is advisable to confirm the pluripotency of hPSCs by their appearance before use. It takes at least 3-4 days for colonies with the typical appearance to emerge after plating at 1,000 cells / cm². When an hPSC culture reaches 80% confluency, the culture must be used or passaged.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Requirement, All differentiation kits

Do I need to sign any license or agreement to use a differentiation kit available through Elixirgen Scientific?

No. You don't need any licence or material transfer agreement (MTA) to use our differentiation kits. However, please be advised that these products are for research use only.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Requirement, All differentiation kits

I was able to harvest hPSCs much more than I needed for the kit use. Can I replate or freeze the leftover hPSCs to maintain their culture?

No, because the instruction given in the protocol is to harvest hPSCs in a differentiation medium.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Protocol, Plating, All differentiation kits

Do you have any videos to show the protocol?

Yes, we have our own YouTube channel. Click "here or search "Elixirgen Scientific" on YouTube. Videos may not cover all the kits but we are working to add more.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Protocol, All differentiation kits

The answer to my question cannot be found in FAQ. What is the best way to contact Elixirgen Scientific if I need immediate assistance?

Please contact our customer support by phone (+1-443-869-5420) or email ([email protected]). We will get back to you as soon as possible.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Inquiry, All differentiation kits

My culture did not survive or had many floating cells/cellular debris on Day 1.

Mixing hPSCs with SeV was done too strongly. Gently rock the plate several times to mix iPSCs with SeV.

Kits: Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR)

Tags: Protocol, SeV, Sendai virus, Plating, Cell death, All Sendai virus(SeV)-based differentiation kits

May I add SeV after hPSCs are plated in all 4 wells?

It is recommended to add SeV into a culture immediately after cells are plated into one well. However, rocking the plate can be done after all 4 wells are treated with SeV.

Kits: Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR)

Tags: Protocol, SeV, Sendai virus, All Sendai virus(SeV)-based differentiation kits

May I mix hPSCs with SeV in a tube and plate them into wells?

No, because hPSCs interacting with SeV are fragile and pipetting during plating will damage them.

Kits: Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR)

Tags: Protocol, SeV, Sendai virus, All Sendai virus(SeV)-based differentiation kits

How can I add such a small volume of SeV dropwise into a culture?

Use a P20 pipettor and make a small drop of SeV solution on the pipet tip as the plunger is slowly pushed down. Since the drop can touch the culture medium, repeat making a drop and touching the medium severall times at different sites within the culture.

Kits: Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR)

Tags: Protocol, SeV, Sendai virus, All Sendai virus(SeV)-based differentiation kits

Will SeV change the genetic information of host hPSCs?

No, because SeV is an RNA virus, does not integrate into the genomic DNA but stay in the cytoplasm.

Kits: Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR)

Tags: Protocol, SeV, Sendai virus, Footprint, All Sendai virus(SeV)-based differentiation kits

Will SeV remain active in the culture?

No, because SeV used in our kits is a temperature-sensitive mutant and active at 33℃ but becomes inactive at 37℃, which is instructed in the protocols.

Kits: Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR)

Tags: Protocol, SeV, Sendai virus, All Sendai virus(SeV)-based differentiation kits

What level of the biosafety containment is required for the use of SeV?

SeV is not pathogenic to humans (i.e., human is not the natural host of the virus) and the infection does not persist in immunocompetent animals. Furthermore, SeV used in our kits do not produce infectious viral particles upon transduction to host hPSCs and is a temperature-sensitive mutant, such that it is active at 33℃ but becomes inactive at 37℃. However, because SeV can be transmitted by aerosol and contact with respiratory secretions and is highly contagious, appropriate care must be taken to prevent the potential mucosal exposure to the virus. Collectively, our SeV-based kits must be used under Biosafety Level 2 (BL-2) containment with a biological safety cabinet or a laminar flow hood, and with appropriate personal protective equipment. In the event that the virus comes into contact with skin or eyes, decontaminate the affected area by flushing with a plenty of water and follow the safety manual prepared by your laboratoy that is approved by the Institutional Biosafety Committee.

Kits: Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV), Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV), Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Trilineage™ Differentiation Kit (EXGS-Q3D), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR)

Tags: Requirement, SeV, Sendai virus, All Sendai virus(SeV)-based differentiation kits

Why do mRNA-based kits require hPSCs maintained under StemFit or StemFlex conditions?

These two culture conditions permit plating hPSCs at a lower density or provide a better cloning efficiency of single hPSCs, and have been producing a high quality differentiation using our mRNA-based kits. Human PSCs maintained under mTeSR1 or E8 conditions are not compatible with our kits and need to be adapted to either StemFit or StemFlex conditions prior to the application to our mRNA-based kits.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Requirement, All mRNA-based differentiation kits

Will the use of mRNA-based kits result in changing the genetic information of host hPSCs?

No, because RNA does not integrate into the genomic DNA but stay in the cytoplasm only for several hours.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Protocol, Footprint, All mRNA-based differentiation kits

Do I need to use any transfection reagent additionally?

No transfection reagent is required additionally.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Requirement, protocol, additional material, All mRNA-based differentiation kits

What kind of RNA is used for differentiation induction?

It is a proprietary formulated RNA and cannot be disclosed.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV), Quick-Muscle™ Skeletal - mRNA Complete Kit (EXGS-QMS), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR), Quick-Tissue™ Endoderm Booster (EXGS-QTEB), Quick-Tissue™ Mesoderm Booster (EXGS-QTMB)

Tags: Contents, All mRNA-based differentiation kits

How long can Coating Material B and C be left in wells?

Coating Material B and C can be left overnight at 37℃.

Kits: Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Cholinergic - Maintenance Medium (EXGS-QNCM), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ Dopaminergic - Maintenance Medium (EXGS-QNDM), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ GABAergic - Maintenance Medium (EXGS-QNGM), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Neuron™ Mixed - Maintenance Medium (EXGS-QNMM), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR)

Tags: Protocol, Coating, Passaging, Quick-Neuron series

What type of glass cover slips can be used for culture?

The use of high-quality German borosilicate glasses is recommended in the text book, "Culturing Nerve Cells”.

Kits: Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Cholinergic - Maintenance Medium (EXGS-QNCM), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ Dopaminergic - Maintenance Medium (EXGS-QNDM), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ GABAergic - Maintenance Medium (EXGS-QNGM), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Neuron™ Mixed - Maintenance Medium (EXGS-QNMM), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR)

Tags: Protocol, Coating, Passaging, Quick-Neuron series

May I skip washing cover slips with PBS after the treatement with Coating Material B or C?

No, because remaining free Coating Material B or C will render subsequent binding of Coating Material C and cells weak.

Kits: Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Cholinergic - Maintenance Medium (EXGS-QNCM), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ Dopaminergic - Maintenance Medium (EXGS-QNDM), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ GABAergic - Maintenance Medium (EXGS-QNGM), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Neuron™ Mixed - Maintenance Medium (EXGS-QNMM), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR)

Tags: Protocol, Coating, Passaging, Quick-Neuron series

I used 150 μl Coating Material B as suggested in the protocol. However, coverslips were covered partially, or Coating Material B did not stay as a drop on top of the cover slip .

Please add 150 μl more Coating Material B and incubate for 2 more hours.

Kits: Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Cholinergic - Maintenance Medium (EXGS-QNCM), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ Dopaminergic - Maintenance Medium (EXGS-QNDM), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ GABAergic - Maintenance Medium (EXGS-QNGM), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Neuron™ Mixed - Maintenance Medium (EXGS-QNMM), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR)

Tags: Protocol, Coating, Passaging, Quick-Neuron series

I found cover slips floating on top of Coating Material B or C. What should I do?

Before starting incubation, please make sure that cover slips are submerged in Coating Material B or C; they can easily be under the liquid by shaking and/or rocking the plate. Alternatively, the cover slips can be flipped since the other side must have been coated.

Kits: Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Cholinergic - Maintenance Medium (EXGS-QNCM), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ Dopaminergic - Maintenance Medium (EXGS-QNDM), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ GABAergic - Maintenance Medium (EXGS-QNGM), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Neuron™ Mixed - Maintenance Medium (EXGS-QNMM), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR)

Tags: Protocol, Coating, Passaging, Quick-Neuron series

I would like to differentiate human PSCs into motor neurons. Do you have such a kit available?

Our Quick-Neuron™ Cholinergic kit will allow you to differentiate motor neurons from human PSCs because motor neurons are derived from cholinergic neurons. Neurons differentiated using the Quick-Neuron™ Cholinergic kit express a motor neuron marker ChAT. Check out the images on the product page.

Kits: Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Cholinergic - mRNA Complete Kit (EXGS-QNC), Quick-Neuron™ Cholinergic - Maintenance Medium (EXGS-QNCM), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ Dopaminergic - mRNA Complete Kit (EXGS-QND), Quick-Neuron™ Dopaminergic - Maintenance Medium (EXGS-QNDM), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ GABAergic - mRNA Complete Kit (EXGS-QNG), Quick-Neuron™ GABAergic - Maintenance Medium (EXGS-QNGM), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF), Quick-Neuron™ Mixed - Maintenance Medium (EXGS-QNMM), Quick-miniBrain™ - SeV neuron and mRNA EC kit (EXGS-QMBSVMR)

Tags: Kit selection, Quick-Neuron series

What would be the expected outcome in results with passaging on Day 3?

Passaged cultures will likely have an almost pure population of neurons, whereas more than 80% of the cultures are expected to be neurons without passaging.

Kits: Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF)

Tags: Protocol, SeV, Sendai virus, Passaging, Sendai virus(SeV)-based Quick-Neuron series

My cultures on Day 1 appeared to have more hPSCs than needed. What should I do?

Please perform passaging on Day 3 as directed in the protocol becuase it is anticipated that the differentiation of neurons is less efficient due to the outgrowth of precursor cells. Passaging on Day 3 will result in enriching neurons in newly prepared cultures. In addition, if you plan to use our kits again, please perform the following test: count and plate 0.1, 0.2, 0.5 and 1.0 x 10^5 cells per well and determine the best cell count that results in a similar cell denstiy to the one shown as the Day 1 image in the protocol.

Kits: Quick-Neuron™™ Cholinergic - SeV Complete Kit (EXGS-QNCSV), Quick-Neuron™™ Cholinergic - SeV Frozen (EXGS-QNCSVF), Quick-Neuron™ Cholinergic - SeV Complete Kit for a 96-well microplate format (EXGS-QNCSV96), Quick-Neuron™ Dopaminergic - SeV Complete Kit (EXGS-QNDSV), Quick-Neuron™ Dopaminergic - SeV Frozen (EXGS-QNDSVF), Quick-Neuron™ GABAergic - SeV Complete Kit (EXGS-QNGSV), Quick-Neuron™ GABAergic - SeV Frozen (EXGS-QNGSVF), Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV), Quick-Neuron™ Mixed - SeV Frozen (EXGS-QNMSVF)

Tags: Protocol, SeV, Sendai virus, Plating, Sendai virus(SeV)-based Quick-Neuron series

What types of neurons will differentiate when Quick-Neuron™ Mixed is used for differentiation induction?

We have tested and confirmed the expression of the following markers: choline acetyltransferase (ChAT, a cholinergic neuron marker), tyrosine hydroxylase (TH, a dopaminergic neuron marker), parvalbumin (PVALB, a GABAergic neuron marker), vesicular glutamate transporter 1 (vGLUT1, a glutamatergic neuron marker), tryptophan hydroxylase 2 (TPH2, a serotonergic neuron marker). The results can be viewed at the product page.

Kits: Quick-Neuron™ Mixed - SeV Complete Kit (EXGS-QNMSV)

Tags: Data, result, Quick-Neuron™ Mixed - SeV Complete Kit

My culture on Day 1 appeared to have more hPSCs than needed. What should I do?

Please go ahead and start QEV treatments as instructed in the protocol. At the end of the Day 1 and Day 2 treatments, perform the puromycin selection using 1.5- or 2-fold more puromycin relative to the optimun concentration determined based on the kill curve.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV)

Tags: Protocol, Plating, Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection

My culture has almost no cells on day 3. What should I do?

The initial plating density was below optimum. If you plan to use our kits again, please plate hPSCs at 4 different counts on Day 0 as follows: 1.6, 1.8, 2.0 and 2.2 x 10^5 cells per well.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV)

Tags: Protocol, Cell death, Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection

My culture has endothelial cells differentiated and becomes almost 100% confluent. Should I passage the culture?

Yes, passaging is highly recommended before a culture reaches 100% confluency. Please check our Quick-Endothelium Vascular Maintenance Medium (Cat #: EXGS-QEVM) and follow the instruction in the protocol.

Kits: Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection (EXGS-QEV)

Tags: Protocol, Passage, Quick-Endothelium™ Vascular - mRNA Complete Kit with optional Drug Selection

My culture has almost no cells but a few spindle-shaped cells differentiated on Day 5 or 6.

The initial plating density was below optimum. If you plan to use our kits again, please increase the number of cells plated on Day 0 to 1.4, 1.6, 1.8, and 2.0 x 10⁵ cells per well.

Kits: Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV)

Tags: Protocol, Cell death, Quick-Muscle™ Skeletal - SeV Complete Kit

My culture has differentiated, spindle-shaped cells but many undifferentiated cells remain at the periphery of the wells on Day 5 or 6.

The initial plating density was above optimum. If you plan to use our kits again, please decrease the number of cells plated on Day 0 to 0.3, 0.4, 0.5 and 0.6 x 10⁵ cells per well.

Kits: Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV)

Tags: Protocol, Low differentiation, Quick-Muscle™ Skeletal - SeV Complete Kit

My culture has differentiated, spindle-shaped cells but many undifferentiated cells remain at the periphery of the wells on Day 5 or 6.

Mesoderm Booster was not used immediately after thawing. Use Mesoderm Booster as instructed.

Kits: Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV)

Tags: Protocol, Low differentiation, Quick-Muscle™ Skeletal - SeV Complete Kit

My culture has differentiated, spindle-shaped cells but only on one side of the well.

QMS-SeV and Mesoderm Booster were not uniformly mixed with the medium. Rock the plate several times gently after addition of QMS-SeV and swirl the plate for 15 seconds after addition of Mesoderm Booster before incubation.

Kits: Quick-Muscle™ Skeletal - SeV Complete Kit (EXGS-QMSSV)

Tags: Protocol, Low differentiation, Quick-Muscle™ Skeletal - SeV Complete Kit

Do I need to use KSR or may I use FBS instead?

The use of FBS will decrease the differentiation efficiency.

Kits: Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV)

Tags: Protocol, Additional materials, Quick-Hepatocyte™ - SeV Complete Kit

My culture has a fewer number of cells plated than the one shown in the protocol on Day 1. Will the differentiation induction work?

The differentiation efficiency will likely decrease. If you plan to use our kits again, please perform the following test: count and plate 1.5, 2.0, 2.5 and 3.0 x 10^5 cells per well and determine the best cell count that results in a similar cell denstiy to the one shown as the Day 1 image in the protocol.

Kits: Quick-Hepatocyte™ - SeV Complete Kit (EXGS-QHSV)

Tags: Protocol, Plating, Quick-Hepatocyte™ - SeV Complete Kit