Quick-Muscle™ Skeletal – SeV Kit

$299.00

Category: Human iPS/ES cell differentiation reagent
Tissue Type: Skeletal Muscle
Scale: 4 wells of 24-well plate

Description

The Quick-Muscle™ Skeletal – SeV Kit facilitates rapid and efficient differentiation of human iPS or ES cells into skeletal muscle cells in just 7 days. Our proprietary transcription factor-based stem cell differentiation method uses a temperature-sensitive Sendai virus to produce a highly pure population of skeletal muscle cells without a genetic footprint. Quick-Muscle™ Skeletal differentiated cell cultures display typical skeletal muscle morphology and markers, such as myosin heavy chain (MHC).

Features

  • Rapid production: Stop waiting weeks. Quick-Muscle™ Skeletal produces skeletal muscle in within a week.
  • Flexible choice: Complete kit includes all the necessary reagents such as media and well coating.
  • No footprint: Other methods leave behind molecular and genetic traces — Quick-Muscle™ Skeletal doesn’t.
  • Easy process: No more complex steps! Quick-Muscle™ Skeletal has a simple and easy-to-understand protocol.

Protocol Outline

 

skeletal muscle protocol
From Day 7, users may maintain differentiated skeletal muscle cells in the maintenance medium best suited for their needs, though we recommend Quick-Muscle™ Skeletal – Maintenance Medium.

Specifications

Scale 4 wells of a 24-well plate
Shipping Conditions Dry ice
Storage Conditions -80°C (some components can be stored at -20°C. See User Guides for more information).

User Guides

SDS/Other

Product Citations

Yanlin Wang, Lei Hao, Hongcai Wang, Katherine Santostefano, Arjun Thapa, John Cleary, Hui Li, Xiuming Guo, Naohiro Terada, Tetsuo Ashizawa, and Guangbin Xia “Therapeutic Genome Editing for Myotonic Dystrophy Type 1 Using CRISPR/Cas9” Molecular Therapy 2018. 26: 2617-2630; doi: 10.1016/j.ymthe.2018.09.003

Reference

Tomohiko Akiyama, Shunichi Wakabayashi, Atsumi Soma, Saeko Sato, Yuhki Nakatake, Mayumi Oda, Miyako Murakami, Miki Sakota, Nana Chikazawa-Nohtomi, Shigeru B. H. Ko, Minoru S. H. Ko. “Transient ectopic expression of the histone demethylase JMJD3 accelerates the differentiation of human pluripotent stem cells.” Development 2016. 143: 3674-3685; doi: 10.1242/dev.139360
Akiyama T, Sato S, Chikazawa-Nohtomi N, Soma A, Kimura H, Wakabayashi S, Ko SBH, Ko MSH (2018). “Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.” Sci Rep. 2018 8(1):1189.

FAQ

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Shipping

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Legal

Not for medical diagnostic or clinical use. For in vitro use only. By use of this product, the recipient agrees to be bound by the terms of this limited use statement. This product may not be further sold or transferred by the recipient and may be used only by the recipient. Elixirgen Scientific makes no representations or warranties of any kind, either expressed or implied, including as to merchantability or fitness for a particular purpose with regards to this product. The terms of this agreement shall be governed under the laws of the State of Maryland, USA.