Category: Human iPS/ES cell differentiation reagent
Tissue Type: Dopaminergic Neurons
Scale: 4 wells of 24-well plate
The Quick-Neuron™ Dopaminergic – mRNA Kit (Small) facilitates rapid and efficient differentiation of human iPS or ES cells into a population of primarily dopaminergic neurons in just 10 days. Our proprietary transcription factor-based stem cell differentiation method uses synthetic mRNAs to produce highly pure populations of neurons without a genetic footprint. Quick-Neuron™ Dopaminergic differentiated cell cultures display typical neurite outgrowth and express a variety of neuronal markers, such as the pan-neuronal marker tubulin beta 3 class III (TUBB3) and the dopaminergic markers tyrosine hydroxylase (TH) and dopamine (DA). When handled and maintained according to the instructions in this user guide, dopaminergic neurons are viable long-term and are suitable for a variety of characterization and neurotoxicity assays.
- Rapid production: Stop waiting weeks. Quick-Neuron™ Dopaminergic produces dopaminergic neurons within 10 days.
- No footprint: Other methods leave behind molecular and genetic traces — Quick-Neuron™ Dopaminergic doesn’t.
- Easy process: No more complex steps! Quick-Neuron™ Dopaminergic has a simple and easy-to-understand protocol.
|Scale||4 wells of a 24-well plate (small) or 6 wells of a 6-well plate/100-mm dish (large)|
|Shipping Conditions||Dry ice|
|Storage Conditions||-80°C (some components can be stored at -20°C. See User Guides for more information)|
- Component N
- Component D4
- Component D5
- Component P
- Coating Material A
- License Statement
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Goparaju, Sravan Kumar, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma, Yukhi Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru B. H. Ko, and Minoru S. H. Ko. “Rapid Differentiation of Human Pluripotent Stem Cells into Functional Neurons by MRNAs Encoding Transcription Factors.” Scientific Reports. Nature Publishing Group, 13 Feb. 2017. Web. 16 Feb. 2017. doi:10.1038/srep42367
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Not for medical diagnostic or clinical use. For in vitro use only. By use of this product, the recipient agrees to be bound by the terms of this limited use statement. This product may not be further sold or transferred by the recipient and may be used only by the recipient. Elixirgen Scientific makes no representations or warranties of any kind, either expressed or implied, including as to merchantability or fitness for a particular purpose with regards to this product. The terms of this agreement shall be governed under the laws of the State of Maryland, USA.